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Cells were then washed 3 times with PBS, then washed with DIPA staining solution for 5 min, followed by glycerol blocking, and then observed using a fluorescence microscope ( 2.7 Statistical analysis Statistical analysis and graph production were performed using GraphPad Prism (GraphPad Software) version 6, and Microsoft Excel was used to perform ANOVA for data analysis

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Results from the phase 2 TROPION-Lung05 trial published in the Journal of Clinical Oncology revealed that among 137 patients given 6 mg/kg of dato-DXd every 21 days for pretreated NSCLC, the confirmed ORR was 35.8% (95% CI, 27.8%-44.4%), including 32.8% of patients experiencing a partial response and 2.9% having a complete response