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Samples were subsequently washed three times for 10 min each with GLF16 diluent (PBS, 2.5% DMSO and 2.5% Tween-20)

[Neurochemical perspectives of the function of monoamine oxidases]

Disclaimer: (*) These statements have not been evaluated by the Federal Drug Administration

This is worth repeating because its so frequently overlooked: even brief, vigorous shaking can be enough to compromise a sample that was otherwise handled correctly at every other stage