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Cell lysate was then diluted with lysate buffer and divided into two aliquotsone treated with C1-27 (100 M) and the other treated with 1% DMSO

Hockenbery, D.M., Oltvai, Z.N., Yin, X., Milliman, C.L

CSF folate levels: The hallmark of CFDS is normal blood folate with low CSF folate, necessitating a lumbar puncture for definitive diagnosisan invasive procedure particularly challenging in children with ASD

100 L of glutathione Sepharose 4B bead slurry (GE17-0756-01) was washed twice with lysis buffer (50 mM Tris, pH 7.4, 150 mM NaCl, 1 mM EDTA, 5 mM 2-mercaptoethanol, 1% Triton X-100) by centrifugation at 100 g for 1 min at 4 C and aspiration of the washing buffer