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Some bacteria produce excessive hydrogen and methane gas during this process

In this study, we firstly confirmed that compared with RBITC-GLP-1RAs@NPs, RBITC-GLP-1RAs@NPs-WYRGRL could effectively target tissues with abundant cartilage, such as joints, and could be retained in cartilage tissues more effectively, and thus be excreted by the kidneys less

Growth retardation and skeletal abnormalities arise from disrupted nucleic acid metabolism and impaired osteoblast activity [5, 6]

Proximity ligation assay (PLA) assay To verify the interaction between SIRT5 and TFR1 in MPC5 cells, the PLA assay was performed as follows: MPC5 cells were fixed with 4% paraformaldehyde for 15 min at room temperature, permeabilized with 0.1% Triton X-100 for 10 min, and blocked with 5% BSA for 30 min at 37 C Cells were then incubated overnight at 4 C with primary antibodies (rabbit anti-SIRT5, 1/1000