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Furthermore, the popliteal lymph node assay was able to discriminate between structurally closely related compounds, for example chemical congeners of D-penicillamine (Hurtenbach et al., 1987), diphenylhydantoin (Kammller & Seinen, 1988) and zimeldine (Thomas et al., 1989, 1991)

Keep peptides according to the manufacturers instructions, generally refrigerated between 2 and 8 degrees Celsius and shielded from light

Kikuchi G, Yoshida T, Noguchi M

After the secondary antibody reaction, the bands were visualized with enhanced chemiluminescence, and the positive pixel area was detected by an image analysis system (Fusion, Germany)