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Wipe the rubber stoppers on both vials with alcohol prep pads

Glutathione exists primarily in two interrelated forms: Reduced glutathione, or GSH: the form able to donate electrons within antioxidant and redox reactions Oxidized glutathione, or GSSG: the form produced after two glutathione molecules form a disulfide bond The body continually recycles oxidized glutathione back into reduced glutathione through enzyme systems that depend partly on cellular energy and nutrient availability

The samples were centrifuged at 20,000 g for 15 min at 4 C

Membranes were again washed three times for 10 min in TBS-T, and developed using WesternBright ECL (Advansta) in myECL Imager (Thermo Scientific) according to the manufacturers instructions