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This finding indicates that the activation of neural orexigenic pathways in response to peripheral ghrelin is not strictly dependent on vagal afferent nerves (74)

The imidazole nitrogen of the histidine side chain donates one of the three nitrogen ligands to the copper, and the position of histidine at the second residue is what enables the deprotonated amide nitrogen of the preceding Gly-His peptide bond to also coordinate the copper