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Copper toxicity: A theoretical concern given the copper content

Beyond direct antioxidant activity, glutathione functions through several enzymatic systems central to detoxification and redox control, including glutathione-S-transferases for conjugation reactions, glyoxylases, glutathione peroxidases and peroxiredoxins for peroxide reduction, and glutaredoxins for thiol-disulfide exchange

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The slides were washed and mounted with Prolong gold anti-fade mountant with DAPI (Thermo Fisher Scientific) and imaged using white light laser Leica SP8 confocal laser scanning microscope