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reported that the low GSH production of permeated cells immobilized using a polyacrylamide gel was caused by the low efficiency of the ATP supply: only 0.5 % of the total regenerated ATP from the glycolytic pathway was actually used for GSH synthesis in E

You might pull the plunger past your target and suck in air, then push back to the right number, unknowingly leaving an air pocket in the middle of the liquid column