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Islet immunohistochemistry Islets were dispersed at 37 C using 0.25% Trypsin-EDTA (Thermo Fisher Scientific, #25200056), plated on Poly-L-lysine pre-coated glass coverslips and fixed with 10% formalin

cool leftovers and refrigerate within 2 hours Thorough cooking : Ensure meat, particularly poultry and minced products, reaches safe internal temperatures (70C for at least 2 minutes or 75C instantaneously)

Overall, oral agents should prioritize glycemic control and -cell preservation while avoiding autoimmunity accelerators (e.g., sulfonylureas)

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