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Over a long period, constant contact can potentially cause compounds from the rubber to leach into the solution, however minor

Decreased mRNA expression or protein levels for TGF1, TGFBR1 [41], SMAD3 [42], SMAD6 [43], and SMAD7 [41, 43] have been reported in more advanced stages of COPD or fibroblasts from COPD patients

Both approaches produce results, so choose based on your schedule and preference