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The resulting protein was centrifuged to remove any aggregates and loaded onto a Superdex 200 16/600 size exclusion chromatography (SEC) column equilibrated in SEC buffer for crystallisation (10 mM Tris-HCl pH 8.0, 200 mM KCl, 0.5 mM PMSF, 1 mM TCEP) 31 or NMR (50 mM mono/dibasic sodium phosphate buffer pH 8.0, 150 mM NaCl, 1 mM TCEP)

How you do it at the individual level is empirical and I think prescriptive, meaning you have to be able to try something, iterate on it, and make a measurement

Current scientific literature continues to explore how GHK-Cu interacts with multiple biological processes, making it an important research compound across several disciplines, including regenerative medicine, biomaterials engineering, and molecular biology

(Czech Republic), one of the leading independent peptide analysis laboratories in Europe