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Studies show that low glutathione is associated with impaired immune responses, while optimizing glutathione status can support certain immune parameters in disease-focused models

For ex vivo activation of liver NH cells, preparations as described above were resuspended in RPMI 1640 media (Gibco, reference 11875) supplemented with 10% FBS, 100 M HEPES (Gibco, 15630106), NEAA (Gibco, 11140050) 100U/ml penicillin/streptomycin (Gibco, 15140122), 1 mM pyruvate, and 3.5 l/L 2-mercaptoethanol and rested at 37C for 30 minutes