CpG Methylase (M. Sssl) Microbial DNA Isolation straightforward

straightforward

Ready-to-use bisulfite conversion reagent used to bisuflite convert purified DNA in the EZ DNA Methylation - Lightning kits

The Zymo-Spin III-P features durable polypropylene construction and a unique silica-based matrix that allows for purification of up to 300 µg of plasmid DNA in ≥ 100 µl eluate when used in combination with ZymoPURE Plasmid Prep buffers

The DNA from both reactions is then amplified using Real-Time PCR

CpG Methylase (M. Sssl) Microbial DNA Isolation straightforwardCpG Methylase completely methylates all cytosines in double stranded, non methylated, and hemimethylated DNA in a 5' CpG 3' context. The recombinant methylase is isolated from an E. coli strain that expresses the methyltransferase gene from Spiroplasma sp. strain MQ1. Reaction conditions have been optimized to maximize the processivity of the enzyme to ensure rapid, complete, and reproducible methylation of DNA for accurate DNA methylation analysis.