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Primary antibodies for Western blotting analysis were rabbit polyclonal anti-GAPDH (Cell Signaling, San Jose, CA) and rabbit anti-EGFR (Santa Cruz Biotechnology)

Am J Clin Nutr 55: 615S619S Apovian CM, Garvey WT, Ryan DH (2015) Challenging obesity: patient, provider, and expert perspectives on the roles of available and emerging nonsurgical therapies

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After 18 hours of treatment, cells were collected and resuspended in sterile PBS before undergoing the following staining with 10 M Liperfluo (Dojindo, #L-248-10) for 30 minutes at 37C