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Thus, cystine limitation suppressed mTORC1 and activated the ISR, the two major amino acid sensing pathways in HepG2 cells

Intracellular ATP content The intracellular ATP content was measured using the ATPlite Luminescence ATP Detection Assay System (Perkin Elmer, Waltham, MA, USA), according to the protocol provided by the manufacturer, and the luminescence was measured using a Tecan Genios microplate reader (Thermo Fisher)

The most frequently reported adverse effects of tirzepatide are predominantly gastrointestinal in nature, reflecting the medication's mechanism of action on GLP-1 receptors in the digestive system