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Triangles are overestimates (i.e., the aliquots taken from the liquid culture at 42 O C did not yield any colonies on agar at 30 O C, so there could not have been more survivors in the liquid culture than values represented by the triangles)

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Taken together, all these results indicate that the newly identified spherical vesicles, with diameters ranging from approximately 3050 nm, may serve in plant retrograde transport and are distinct from the tubular recycling endosomes described in animal systems