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ThermoFisher, 11320033) with 2 mM L-glutamine, 10 mM HEPES (Sigma-Aldrich, H3375-25g), 50 U ml 1 penicillinstreptomycin, 1 B27 (ThermoFisher, 12587-010), 1 N-2 (ThermoFisher, 17502-048), 50 ng ml 1 EGF (Peprotech, AF-100-15) and 100 ng ml 1 Noggin (Peprotech, 250-38)