Role of STAT3 signaling pathway in breast cancer
After blocking in 5% skimmed milk powder for 2 hours at room temperature, the samples were incubated overnight at 4C with primary antibodies, including ERK1/2 (#9102), p-ERK1/2 (#9101), JNK1/2 (#9252), p-JNK (#9251), p38 MAPK (#9212), and p-p38 MAPK (#9211), which were obtained from Cell Signaling Technology (Beverly, MA, USA), as well as VEGF-a (sc-7269) and Tubulin (sc-8035), which were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA)
JT: Conceptualization, Data curation, Formal Analysis, Investigation, Methodology, Resources, Software, Supervision, Validation, Visualization, Writing original draft, Writing review and editing
Compared to mice given free GSH, whey encapsulated GSH resulted in significantly increased antioxidant capacity in the plasma through the entire period of study (from 15 minutes until 240 minutes after ingestion).* Non-toxic In the 28-day toxicology arm of the study, a diet containing up to 4% GSH in a whey protein matrix was found to be safe and non-toxic
