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Before the operation, the patient needs to stand on tiptoes to contract the calf muscles, thereby accurately locating the free subcutaneous fat layer

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antioxidants including Glutathione ethyl ester (Cayman Chemical Company 14953), 2,2,6,6-Tetramethylpiperidine 1-oxyl (TEMPO, Sigma-Aldrich 214000-1 G), mitochondrial-targeted TEMPO (mitoTEMPO, Sigma-Aldrich SML0737-25MG), mitoCoQH2 (Mitoquinol, Cayman Chemical Company CAY89950), and CoQ10 (Coenzyme Q10, Sigma-Aldrich C9538)