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Highly oxidative organs such as the heart, liver, and brain are particularly vulnerable to pathogen-induced mitochondrial dysfunction due to their dependence on oxidative phosphorylation

After the mixture was allowed to cool down to the ambient temperature, it underwent centrifugation and was then subjected to three rounds of washing using ethanol and water

Following the injection, in vivo imaging was conducted using an IVIS Spectrum (PerkinElmer) at various time points: 1 h, 2 h, 4 h, 6 h, 8 h, 12 h, 24 h, and 48 h post-injection, utilizing an excitation wavelength of 780 nm and a fluorescence emission wavelength of 840 nm

As shown in Supplementary Figure 1, we did not observe a significant difference in the cell number between the control and ABT263 treatment group