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Description

World J Gastroenterol (2008) 14:5990

Time frame and selection criteria: The focus was primarily on articles published in the last two decades, with particular attention to comprehensive reviews, landmark studies, and significant clinical trials

Generation of YPEL5 -mutant cells HEK293T cells were transfected with either pN106/YPEL5-FLAG-WT, pN106/YPEL5-FLAG-T36A, pN106/YPEL5-FLAG-R41A or pN106/YPEL5-FLAG-L62A, along with pMD2.G (Addgene, 12259) and psPAX2 (Addgene, 12260), using TransIT-LT1 transfection reagents (Mirus Bio) to produce lentivirus

TP53, a tumor suppressor protein known as the 'guardian of the genome,' plays a crucial role in maintaining genomic stability and regulating cell cycle progression