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Relative mRNA expression was determined using the GoTaq 1-step real-time quantitative PCR (RT-qPCR) system (TAKARA) and qPCR using SYBR premix Ex Taq kit (TAKARA), and RT-qPCR was performed on an ABI Prism 7500 Fast Real-Time PCR System (Applied Biosystems, CA, USA)

Vitamin B12 plays a significant role in several body functions, including red blood cell production

Allies of Skin describes it as an amino acid complex that can help improve signs of aging, such as enhancing skin density and firmness. Though its unclear, this ingredient may be based on the branded ingredient Neodermyl, as most sources point to it when investigating copper lysinate in skin care

Methylcobalamin is often preferred due to its higher bioavailability and direct usability