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They do not clearly define the role of lysosomal activity, autophagy or protease changes in microgravity-induced bone loss, muscle atrophy, apoptosis or oxidative stress

Samples were diluted in 6-salt PBS with or without GSH to twice the concentration required to obtain an OD of 1.0 in D antigen ELISA, duplicate samples heated for 10 min at each temperature were then diluted 1:1 with 2% dried milk in 6-salt PBS and cooled on ice

Hepatic TG and cholesterol contents were measured using commercially available kits (Wako Pure Chemical Industries, Osaka, Japan)