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The sections were blocked for 1 h at room temperature with 5% normal goat serum and stained with anti-atrial natriuretic peptide (ANP) antibody (Servicebio, Wuhan, China) diluted 1: 200, anti-myosin heavy chain 7(MYH7) antibody (Proteintech Group, Inc, Wuhan, China) diluted 1: 200, anti-cytochrome c oxidase subunit II (COX II) antibody (Cell Signaling Technology, Inc, Boston, USA) diluted 1: 1000, anti-NLRP3 antibody diluted (ABclonal, Wuhan, China) 1: 200, and anti- interleukin-18 (IL-18) antibody (Servicebio, Wuhan, China) diluted 1: 1000 overnight at 4 C

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SESN pathway in MAFLD SESN, a family of highly conserved proteins, which can be induced by various stress reaction like DNA damage and oxidative stress, actively participates in reactive oxygen species (ROS) and mechanistic target of rapamycin complex 1 (mTORC1) signaling pathways [15]

Sperm counts have dropped worldwide