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Methods Peptide synthesis All peptides were prepared using ChemMatrix Rink amide resin and Fmoc-based automated peptide synthesis on a Prelude X peptide synthesizer (Gyros Protein Technologies) with induction heating and oscillation mixing

Through studies of mice and rat models, it has been determined that PPG neurons contain projection tracts to various locations on the brain, including primarily the hypothalamus, supraoptic nucleus, and autonomic regions such as the limbic forebrain, portions of the mesolimbic reward system, and other regions involved in learning and memory [47, 50]

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