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The anti-metabolite drug MTX is frequently used to treat cancer and inhibit the immune system

Zietz A, Gorey S, Kelly PJ, et al

Gene essential analysis and in silico drug prediction In silico essential drug prediction was performed on all models using a modified version of the singleGeneDeletion function from the COBRA toolbox [28] and the biomass and ATP production as an objective function for cancer and control models, respectively